An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens |
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| Authors: | Thomson Lynn M Traore Hamidou Yesilkaya Hasan Doig Christine Steingrimsdottir Herdis Garcia Lucia Forbes Ken J |
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| Institution: | Department of Medical Microbiology, University of Aberdeen, U.K. |
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| Abstract: | A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. |
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