Silencing of HIV by hairpin-loop-structured DNA oligonucleotide |
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| Authors: | Moelling Karin Abels Susanne Jendis Joerg Matskevich Alexey Heinrich Jochen |
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| Institution: | Institute of Medical Virology, University of Zurich, Switzerland. moelling@immv.unizh.ch |
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| Abstract: | We describe inhibition of HIV replication by a partially double-stranded 54mer oligodeoxynucleotide, ODN, which consists of an antisense strand targeting the highly conserved polypurine tract, PPT, of HIV, and a second strand, compatible with triple-helix formation. Upon treatment of HIV-infected cells with ODN early after infection no viral nucleic acids, syncytia or p24 viral antigen expression was observed. The ODN-mediated effect was highly sequence-specific. The ODN against HIV-IIIB was effective preferentially against its homologous PPT and less against the PPT of HIV-BaL differing in two of 24 nucleotides and vice versa. It may be interesting mechanistically as an antiviral drug. |
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