Localized Aurora B activity spatially controls non-kinetochore microtubules during spindle assembly |
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Authors: | Marvin?E?Tanenbaum Email author" target="_blank">René?H?MedemaEmail author |
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Institution: | (1) Department of Medical Oncology and Cancer Genomics Centre, University Medical Center Utrecht, Universiteitsweg 100, Str.2.118, 3584 CG Utrecht, The Netherlands; |
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Abstract: | Efficient spindle assembly involves the generation of spatial cues around chromosomes that locally stabilize microtubule (MT)
plus-ends. In addition to the small GTPase Ran, there is evidence that Aurora B kinase might also generate a spatial cue around
chromosomes but direct proof for this is still lacking. Here, we find that the Aurora B substrate MCAK localizes to MT plus-ends
throughout the mitotic spindle, but its accumulation is strongly reduced on MT plus-ends near chromatin, suggesting that a
signal emanating from chromosomes negatively regulates MCAK plus-end binding. Indeed, we show that Aurora B is the kinase
responsible for producing this chromosome-derived signal. These results are the first to visualize spatially restricted Aurora
B kinase activity around chromosomes on an endogenous substrate and explain how Aurora B could spatially control the dynamics
of non-kinetochore MTs during spindle assembly. |
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Keywords: | |
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