Augmentation of antigen-specific immune responses using DNA-fusogenic liposome vaccine |
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| Authors: | Yoshikawa Tomoaki Imazu Susumu Gao Jian-Qing Hayashi Kazuyuki Tsuda Yasuhiro Shimokawa Mariko Sugita Toshiki Niwa Takako Oda Atushi Akashi Mitsuru Tsutsumi Yasuo Mayumi Tadanori Nakagawa Shinsaku |
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| Institution: | Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Osaka University, Japan. |
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| Abstract: | In an attempt to enhance the immunological efficacy of genetic immunization, we investigated a new biological means for delivering antigen gene directly to the cytoplasm via membrane fusion. In this context, we investigated fusogenic liposome (FL) encapsulating DNA as a possible genetic immunization vehicle. RT-PCR analysis indicated that a FL could introduce and express encapsulating OVA gene efficiently and rapidly in vitro. Consistent with this observation, an in vitro assay showed that FL-mediated antigen-gene delivery can induce potent presentation of antigen via the MHC class I-dependent pathway. Accordingly, immunization with FL containing the OVA-gene induced potent OVA-specific Th1 and Th2 cytokine production. Additionally, OVA-specific CTL responses and antibody production were also observed in systemic compartments including the spleen, upon immunization with the OVA-gene encapsulating FL. These findings suggest that FL is an effective genetic immunization carrier system for the stimulation of antigen-specific immune responses against its encoding antigen. |
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| Keywords: | DNA vaccine Tumor vaccine Liposome Drug delivery system |
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