Heterologous complementation of the Klaac null mutation of Kluyveromyces lactis by the Saccharomyces cerevisiae AAC3 gene encoding the ADP/ATP carrier |
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Authors: | Fontanesi Flavia Viola Anna Maria Ferrero Iliana |
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Institution: | Department of Genetics, Anthropology & Evolution, University of Parma, Parco Area delle Scienze, Parma, Italy. |
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Abstract: | The KlAAC gene, encoding the ADP/ATP carrier, has been assumed to be a single gene in Kluyveromyces lactis, an aerobic, petite-negative yeast species. The Klaac null mutation, which causes a respiratory-deficient phenotype, was fully complemented by AAC2, the Saccharomyces cerevisiae major gene for the ADP/ATP carrier and also by AAC1, a gene that is poorly expressed in S. cerevisiae. In this study, we demonstrate that the Klaac null mutation is partially complemented by the ScAAC3 gene, encoding the hypoxic ADP/ATP carrier isoform, whose expression in S. cerevisiae is prevented by oxygen. Once introduced into K. lactis, the AAC3 gene was expressed both under aerobic and under partial anaerobic conditions but did not support the growth of K. lactis under strict anaerobic conditions. |
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Keywords: | Kluyveromyces lactis heterologous complementation ScAAC3 ADP/ATP carrier semi-anaerobiosis anaerobiosis |
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