Isolation of constitutive mutants for L-arabinose utilization in Bacillus subtilis. |
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| Authors: | I S -Nogueira, H Paveia, H de Lencastre |
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| Affiliation: | Laboratório de Genética Molecular, Instituto Gulbenkian de Ciência, Oeiras, Portugal. |
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| Abstract: | Constitutive mutants for L-arabinose utilization were isolated from Bacillus subtilis 168T+ and showed resistance to D-fucose, a nonmetabolizable analog of L-arabinose. The mutations that conferred the constitutive phenotype (Arac) were mapped between cysB and hisA. All the mutants showed an isomerase activity which was reduced to 50 to 70% in the presence of L-arabinose and to 10% in the presence of glucose. |
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