Purification of human serum amyloid P component (SAP) by calcium affinity chromatography |
| |
| Authors: | T Mantovaara H Pertoft J Porath |
| |
| Institution: | Institute of Biochemistry, University of Uppsala, Sweden. |
| |
| Abstract: | Serum amyloid P component (SAP) has been purified from human serum by means of immobilized metal ion affinity chromatography (IMAC). It was selectively concentrated on carboxymethylated aspartic acid agarose (CM-Asp-agarose) loaded with calcium and, employing very mild conditions, purified to electrophoretical and immunological homogeneity in a single step amounting to about 1900-fold purification. As a purification method our procedure thus compares well with bio-specific affinity chromatography. |
| |
| Keywords: | |
|
|
