Optimization and use of a spectrophotometric method for determining polysaccharides in <Emphasis Type="Italic">Echinacea purpurea</Emphasis> |
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Authors: | Nina Ko?evar Glava? Iztok Jo?e Ko?ir Janko Rode Samo Kreft |
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Institution: | 1.Department of Pharmaceutical Biology,University of Ljubljana, Faculty of Pharmacy,Ljubljana,Slovenia;2.The Institute for Hop Research and Brewing,?alec,Slovenia;3.Agriculture and Forestry Chamber of Slovenia,Ljubljana,Slovenija |
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Abstract: | Echinacea purpurea is one of the most widely used immunostimulant plants. Its main active compounds are polysaccharides, glycoproteins, caffeic
acid derivatives, alkamides, and melanins. The article describes an optimized extraction procedure that enables spectrophotometric
quantification of polysaccharides from Echinacea purpurea. The extraction procedure can be widely applied as it demonstrated to be useful for determining polysaccharide content in
flowers and leaves, in summer and autumn plants, in plants with green and red stem, and in plants from two different plantations.
A significantly higher content of polysaccharides in flowers in comparison to leaves, as well as in plants with green stems
in comparison to plants with red stems was determined. Statistical differences were absent in plants collected in different
seasons and growing at different plantations. |
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Keywords: | |
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