Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica |
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Authors: | Eymar E Alegre J Toribio M López-Vela D |
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Institution: | 1.Dpto. Química Agrícola, Geología y Geoquímica, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain ;2.Instituto Madrile?o de Investigación Agraria y Alimentaria (IMIA), Finca ‘El Encín’, Apdo. 127, 28800, Alcalá de Henares, Madrid, Spain ; |
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Abstract: | A sterile hydroponic culture system suitable for studying nitrogen (N) uptake ofLagerstroemia indica L.in vitro was developed. Four different treatments were assayed: with and without activated charcoal (AC and NAC, respectively), with
and without 50 μM of 6-benzyladenine (+BA and −BA, respectively). Medium pH, electrical conductivity (EC), NO3
− and NH4
+ concentrations were measured weekly. At the end of the culture, propagules were sampled and SPAD indices, and shoot and root
fresh weights were determined. Explants grown in media with activated charcoal were able to take up both NO3
− and NH4
+, although NH4
+ uptake was lower. Subsequently the pH of the media was maintained between 5.5–6.0. In treatments with no addition of activated
charcoal, NH4
+ uptake was preferential and the pH dropped to 3.1. Explants in these conditions were unable to raise the pH by taking up
NO3
−, especially when root morphogenesis was inhibited by addition of BA. Supply of this PGR produced root growth inhibition,
which was almost complete in the treatment without activated charcoal. This component significantly reduced the inhibitory
effect of 50 μM BA on root growth.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | ammonium (NH4 +) hydroponic culture nitrate (NO3 − ) nutrient uptake plant nutrition sterile conditions |
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