Identification and characterization of microRNAs from the bovine adipose tissue and mammary gland |
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Authors: | Gu Zhiliang Eleswarapu Satyanaryana Jiang Honglin |
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Institution: | Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 3130 Litton Reaves Hall, Blacksburg, VA 24061-0306, USA. |
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Abstract: | We report the identification of bovine miRNAs by cloning small RNAs from adipose tissue and the mammary gland. Fifty-nine distinct miRNAs were identified, five of them were not homologous to known mammalian miRNAs, and many of them had 3' and/or 5' end variants. Ribonuclease protection assays indicated that miR-23a and miR-24, whose genes are closely located on the same chromosome, were co-expressed in different tissues. The assays also suggested a role for several miRNAs in the mammary gland and a role for miR-133, a previously known skeletal and cardiac muscle-specific miRNA, in the rumen, an organ unique to the ruminant. |
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Keywords: | MicroRNA Bovine Adipose Mammary gland Rumen |
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