Blue fluorescent antibodies as reporters of steric accessibility in virus conjugates |
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Authors: | Wang Qian Raja Krishnaswami S Janda Kim D Lin Tianwei Finn M G |
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Affiliation: | Department of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA. |
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Abstract: | Nonenveloped viruses provide the chemist with large, preassembled polyvalent protein scaffolds for modification. These structures are typically porous to small molecules but not to large ones. The solution-phase structures and reactivities of such assemblies may be substantially different than indicated by X-ray crystal structures. Here, the attachment of organic compounds to either the inside or outside surface of the cowpea mosaic virus (CPMV) coat protein was verified with an indicating antibody-antigen interaction. Antibody binding was subsequently blocked by the installation of poly(ethylene glycol) chains. These results typify the type of site-specific control that is available with CPMV and related virus building blocks. |
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