Limited genetic diversity in <Emphasis Type="Italic">Salmonella enterica</Emphasis> Serovar Enteritidis PT13 |
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| Authors: | Adam B Olson Ashleigh K Andrysiak Dobryan M Tracz Jean Guard-Bouldin Walter Demczuk Lai-King Ng Anne Maki Frances Jamieson Matthew W Gilmour |
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| Institution: | (1) National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada;(2) United States Department of Agricultural, Agricultural Research Service, Athens, GA, USA;(3) Ontario Central Public Health Laboratory, Ministry of Health and Long-Term Care, Toronto, ON, Canada;(4) Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada |
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| Abstract: | Background
Salmonella enterica serovar Enteritidis has emerged as a significant foodborne pathogen throughout the world and is commonly characterized by
phage typing. In Canada phage types (PT) 4, 8 and 13 predominate and in 2005 a large foodborne PT13 outbreak occurred in the
province of Ontario. The ability to link strains during this outbreak was difficult due to the apparent clonality of PT13
isolates in Canada, as there was a single dominant pulsed-field gel electrophoresis (PFGE) profile amongst epidemiologically
linked human and food isolates as well as concurrent sporadic strains. The aim of this study was to perform comparative genomic
hybridization (CGH), DNA sequence-based typing (SBT) genomic analyses, plasmid analyses, and automated repetitive sequence-based
PCR (rep-PCR) to identify epidemiologically significant traits capable of subtyping S. Enteritidis PT13. |
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