| 中国东方铃蟾(Bombina orientalis)皮肤中C_(-12-b)肽的分离与鉴定 |
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| 引用本文： | 陈枫,赵轶千.中国东方铃蟾(Bombina orientalis)皮肤中C_(-12-b)肽的分离与鉴定[J].生理学报,1987,39(2):202-206. |
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| 作者姓名： | 陈枫 赵轶千 |
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| 作者单位： | 同济医科大学生理教研室
(陈枫)，同济医科大学生理教研室(赵轶千) |
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| 摘 要： | 东方铃蟾鲜皮的甲醇提取液,可引起大鼠离体平滑肌的收缩。此液经碱性氧化铝柱层析分离,其80%乙醇洗脱的 C 组分显示生物活性。C 组分再经葡聚糖凝胶 G-15柱分离,其活性较强的早期洗脱组分 C_(_12),可被糜蛋白酶水解灭活,但其活性并不被5-HT 拮抗剂赛庚啶2×10~(-6)mol/L]完全拮抗。继用反相高效液相色谱(RP-HPLC)分析,见分离出的 C_(_12_h)峰与铃蟾肽~*(Bombesin,BBS)的出峰时间相同,且具相同的氨基酸组成。上述实验结果提示,C_(_12_h)肽可能就是铃蟾肽。
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| 关 键 词： | 东方铃蟾 C_(12_h)肽 铃蟾肽 |
ISOLATION AND IDENTIFICATION OF C_(-12-h) PEPTIDE FROM THE SKIN OF Bombina orientalis IN CHINA |
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| CHEN FENG ZHAO YI-QIAN.ISOLATION AND IDENTIFICATION OF C_(-12-h) PEPTIDE FROM THE SKIN OF Bombina orientalis IN CHINA[J].Acta Physiologica Sinica,1987,39(2):202-206. |
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| Authors: | CHEN FENG ZHAO YI-QIAN |
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| Abstract: | Methanol extracts of the fresh skin of China amphibians (Bombina orientalis)caused contraction of the smooth muscles of rat in vitro.The crude extracts wereisolated by column chromatography of alkaline alumina,and a bioactive fractionwas obtained from the 80% ethanol eluate.Submitting the fraction on SephadexG-15 column,we found that the early eluate C_(-12),showing more bioactivity,was inactivated by hydrolysis with chymotrypsin,but not completely antagonizedby cyproheptadine.C_(-12)was further analyzed by reversed phase-high perfor-mance liquid chromatography (RP-HPLC).The elution time of C_(-12-h) peak wasthe same as that of bombesin,and these two peptides had the same amino acid com-position.These experimental results suggest that C_(-12-h) peptide is most pro-bably bombesin. |
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| Keywords: | Bombina orientalis C_(-12-h) peptide bombesin |
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