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Restriction endonuclease BamHI cleaves bacteriophage Mu DNA within cistrons E and F |
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| Authors: | J A Engler R A Forgie M M Howe |
| Institution: | 1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724 U.S.A.;1. Department of Bacteriology, University of Wisconsin, Madison, WI 53706 U.S.A. |
| Abstract: | We have cleaved phage Mu DNA with restriction endonucleases EcoRI and BamHI and have cloned three specific DNA fragments from the middle of the Mu genome into vector plasmid pBR322. By marker rescue experiments, we have determined that the two BamHI cleavage sites in Mu DNA occur within cistrons E and F. |
| Keywords: | (Cloning of Mu DNA pBR322 vector plasmid mapping by marker rescue recombinant DNA) am amber amp ampicillin pfu plaqueforming units tet tetracycline |
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