| 反相高效液相色谱法直接检测芳香族氨基酸 |
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| 引用本文： | 曲冬梅,弓爱君,高鹤永,邱丽娜. 反相高效液相色谱法直接检测芳香族氨基酸[J]. 氨基酸和生物资源, 2004, 26(4): 71-73. DOI: 10.3969/j.issn.1006-8376.2004.04.024 |
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| 作者姓名： | 曲冬梅 弓爱君 高鹤永 邱丽娜 |
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| 作者单位： | 北京科技大学应用科学学院,100083 |
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| 摘 要： | 用反相高效液相色谱-变波长紫外检测法直接测定了芳香族氨基酸。流动相为磷酸盐缓冲溶液(pH6)和甲醇的混合溶液,其体积配比为7030,流速为0.6ml/min,变波长紫外检测,10min内完成。酪氨酸、苯丙氨酸、色氨酸的线性范围分别为50～500μg·mL-1、200～20000μg·mL-1、20～2000μg·mL-1,三者的检测限分别为10μg·mL-1、150μg·mL-1、5μg·mL-1。
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| 关 键 词： | 高效液相色谱 紫外检测 苯丙氨酸 酪氨酸 色氨酸 |
| 文章编号： | 1006-8376(2004)04-0071-03 |
| 修稿时间： | 2004-06-17 |
Determination of Aromatic Amino Acids by HPLC with UV Detection at Changeable Wavelength |
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| QU Dong-mei,GONG Ai-jun ,GAO He-yong,QIU Li-na. Determination of Aromatic Amino Acids by HPLC with UV Detection at Changeable Wavelength[J]. Amino Acids & Biotic Resources, 2004, 26(4): 71-73. DOI: 10.3969/j.issn.1006-8376.2004.04.024 |
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| Authors: | QU Dong-mei GONG Ai-jun GAO He-yong QIU Li-na |
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| Affiliation: | QU Dong-mei,GONG Ai-jun *,GAO He-yong,QIU Li-na |
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| Abstract: | A method for determination of aromatic amino acids by RP-HPLC with UV detection at changeable wavelength was developed . Chromatographic conditions were as follows: mobile phase of phosphate buffer (pH6.0) and methanol in a ratio of 70: 30; flow rate of 0.6 ml/min. Samples were finally analyzed in 10min. Linear range of tyrosine, phenylalanine and tryptophane was 50-500μg·mL -1 , 200-20000μg·mL -1 and 20-2000μg·mL -1 . Their detection limit was 10μg·mL -1 、150μg·mL -1 and 5μg·mL -1 . |
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| Keywords: | high performance liquid chromatography (HPLC) UV detection phenylalanine tyrosine tryptophane |
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