Crystallization of bacteriorhodopsin solubilized by a tripod amphiphile |
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Authors: | Theisen Michael J Potocky Terra B McQuade D Tyler Gellman Samuel H Chiu Mark L |
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Institution: | Department of Structural Biology, Abbott Laboratories, Abbott Park, Illinois 60064-6098, USA. |
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Abstract: | Bacteriorhodopsin (bR) is solubilized efficiently as a monomer by a novel surfactant, a tripod amphiphile (TPA), which permits the formation of purple hexagonal bR crystals under several conditions. The crystals, although small, diffract to 2.5 A resolution using synchrotron radiation. TPA may be useful for the solubilization, purification, and crystallization of other membrane proteins. |
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