Different effects of inorganic and triethyl lead on growth and ultrastructure of lily pollen tubes |
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Authors: | G Röderer H -D Reiss |
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Institution: | (1) Present address: Fraunhofer-Institut für Umweltchemie und Ökotoxikologie, Grafschaft, Postfach 1620, D-5948 Schmallenberg, Germany;(2) Institut für Botanik, Universität Hohenheim, Stuttgart;(3) Zellenlehre, Universität Heidelberg, Heidelberg |
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Abstract: | Summary Pollen tubes ofLilium longiflorum growingin vitro were treated for 1 h with inorganic lead (Pb) and with triethyl lead (TriEL) and studied by light and electron microscopy. Pb was considerably more toxic in relation to inhibition of pollen tube growth (EC50=6 M Pb) than was TriEL (EC50=60 M TriEL). On the other hand, at almost the entire concentration range tested (25-500 M) TriEL caused aberrant tubes and tube swellings. Pb did not cause tube swellings, even at highly growth-impairing concentrations. Pb (60 M) predominantly affected the ultrastructure of the growing cell walls without impairing the distribution of the cell organelles in the tube tips. In contrast, 50 and 100 M TriEL did not visibly influence cell wall ultrastructure but it severely damaged dictyosomes; 100 M TriEL also disturbed the original order of cell organelles in the tube tips. Cortical microtubules were selectively and completely destructed by TriEL at concentrations (50 M) where no effect on polar organization of the tube tips occurred but they remained unimpaired by 60 M Pb, indicating selective and effective interaction of TriEL with these cell organelles.Abbreviations EC50
effective lead concentration causing 50% inhibition of pollen tube growth
- MTs
microtubules
- Pb
inorganic lead
- TriAL
trialkyl lead
- TriEL
triethyl lead |
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Keywords: | Lead toxicity Lilium Microtubules Pollen tubes Tip growth Triethyl lead |
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