| 用聚焦层析法纯化人巯基蛋白酶抑制肽C-hCRI_c |
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| 引用本文： | 孙诠,汪淑荣,姜春霞,冯建芳,李平,陈明哲.用聚焦层析法纯化人巯基蛋白酶抑制肽C-hCRI_c[J].中国生物化学与分子生物学报,1992,8(5):593-596. |
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| 作者姓名： | 孙诠 汪淑荣 姜春霞 冯建芳 李平 陈明哲 |
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| 作者单位： | 北京医科大学第三医院心血管研究室，北京医科大学第三医院心血管研究室，北京医科大学第三医院心血管研究室，北京医科大学第三医院心血管研究室，北京医科大学第三医院心血管研究室，北京医科大学第三医院心血管研究室 北京 100083，北京 100083，北京 100083，北京 100083，北京 100083，北京 100083 |
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| 摘 要： | 以肾衰病人尿为材料,通过碱变性、酸变性和热变性除去部分杂质和尿蛋白。然后用羧甲基-木瓜蛋白酶-琼脂糖4B亲和层析,最后用多缓冲剂聚焦层析,把人的巯基蛋白酶抑制肽C(human cysteine proteinase inhibitor C,简称 hCPI_C)和其他的hCPI_C分开,制备得高纯度、高活性的hCPI_C。在SDS-PAGE中为均一条带,每mg蛋白可抑制50单位木瓜蛋白酶活性的50-63％。
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| 关 键 词： | 人巯基蛋白酶抑制肽 C 聚焦层析 亲和层析 组织蛋白酶 |
| 收稿时间： | 1992-10-20 |
Purification of Human Cysteine Proteinase Inhibitor C (hCPIc) by Chromatofocusing |
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| Sun,Quan Wang,Shu-rong Jiang,Chun-xia Feng,Jian-fangLi,Ping Chan,Ming-zhe.Purification of Human Cysteine Proteinase Inhibitor C (hCPIc) by Chromatofocusing[J].Chinese Journal of Biochemistry and Molecular Biology,1992,8(5):593-596. |
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| Authors: | Sun Quan Wang Shu-rong Jiang Chun-xia Feng Jian-fangLi Ping Chan Ming-zhe |
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| Institution: | (Cardiovascular Research Laboratory,The Third Hospital,Beijing Medical University,Beijing 100083 |
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| Abstract: | Human cysteine proteinase inhibitor C has been purified by precipitating part of urine protein with acid, base and heat denaturation followed by, Cm-papain-sepharose 4B affinity chromatography, and finally using polybuffer exchanger 94 chromatofocusing to separate hCPIc from other hCPI.The preparation is high in purity with high inhibitory activity against papain.The hCPIc prepared exhibits on ly one band of protein on SDS-PAGE 1mg of this protein preparation is able to inhibits 50-60% of the proteinase activity in 50 BAEE units of papain. |
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| Keywords: | Human Cysteine Proteinase Inhibitor C Chromatofocusing Affinity Chro-matography Cathepsin |
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