肛门生殖器区肿瘤组织中HPV的快速检测和分型

迄今已发现的人乳头瘤病毒（ＨＰＶ）型已超过７７型，其中至少有３０个型与泌尿生殖道肿瘤有关。所以ＨＰＶ的检测与分型对于泌尿生殖道肿瘤的病因和预后将是非常重要的。ＨＰＶ有很大的异质性，故用常规的诊断技术来测定未知标本中的ＨＰＶ基因型有一定困难。为此，我们应用了反向点杂交法（ＲＯＢ）来检测ＨＰＶ分型。即用７种序列特异性寡核苷酸探针分别对应７型ＨＰＶ（ＨＰＶ６Ｂ、１１、１６、１８、３１、３３、３５），这些

RAPID DETECTION AND GENOTYPING OF HUMAN PAPILLOMAVIRUS IN THE TUMOR TISSUE OF THE ANO-GENITAL REGION

More than 77 types of human papillomavirus (HPV) have been found up to now, among which at least 30 types are associated with uro-genital tumors. So the detection and genotyping would be of great importance for studying the etiology and prognosis of the uro-genital tumors. However, HPV has great heterogeneity, so the detection of HPV type in an unknown specimen with routine diagnostic techniques would be somewhat difficult. Hence we used the reverse dot blot (RDB) technique to detect and genpotype the infecting HPVs. Seven sequence-specific oligonucleotide probes, corresponding respectively to 7 types of HPV(HPV6B, 11,16,18,31,33,,and 35) were used. After the synthesis of these 7 types of probes, they were fixed successively in order on a strip of nylon membrane to form 7 spots, then they were hybridized with the PCR products of the specimen DNA sequences. The result is that any one of the 7 types of HPV DNA, if positive, can be identified on one nylon strip. We collected 177 specimens from the hospitals in Guangzhou (except that 20 cases of esophageal carcinoma were from Henan Province). RDB technique was used to detect and genotype the infecting HPVs and the results were as follows: Uterine cervical carcinoma 86 cases, including 68 squamous carcinomas and 18 adenocarcinomas, the HPV positive numbers were 59/68 (86.76%) of the former (47 for type 16, 9 for type 18, 3 for type 16, 18 mixed infection) and 8/18 (44.44%) of the latter (5 for type 18, 3 for type 16); condyloma acuminatum 42 cases with 39 HPV positive ones (92.86%) of which 17 were type 6B, 20 of type 11 and 2 of mixed infection of types 6B and 11;chronic cervicitis 29 cases, with only one HPV positive (3.45%); and finally esophageal carcinoma 20 cases, with 7 HPV positive ones (35.00%).