Isolation of DNA sequences on human chromosome 21 by application of a recombination-based assay to DNA from flow-sorted chromosomes |
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| Authors: | Umadevi Tantravahi Gordon D. Stewart Margaret Van Keuren Gerard McNeil Sayon Roy David Patterson Harry Drabkin Marc Lalande David M. Kurnit Samuel A. Latt |
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| Affiliation: | (1) Genetics Division, The Children's Hospital, 300 Longwood Avenue, 02115 Boston, MA, USA;(2) Mental Retardation Center, The Children's Hospital, 02115 Boston, MA, USA;(3) Howard Hughes Medical Institute at The Children's Hospital, 02115 Boston, MA, USA;(4) Department of Pediatrics, Harvard Medical School, 02115 Boston, MA, USA;(5) Department of Genetics, Harvard Medical School, 02115 Boston, MA, USA;(6) Eleanor Roosevelt Institute for Cancer Research, University of Colorado Health Sciences Center, 80206 Denver, CO, USA;(7) Howard Hughes Medical Institute at the University of Michigan Medical Center, 48109 Ann Arbor, MI, USA |
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| Abstract: | Summary By merging two efficient technologies, bivariate flow sorting of human metaphase chromosomes and a recombination-based assay for sequence complexity, we isolated 28 cloned DNA segments homologous to loci on human chromosome 21. Subregional mapping of these DNA segments with a somatic cell hybrid panel showed that 26 of the 28 cloned DNA sequences are distributed along the long arm of chromosome 21, while the other 2 hybridize with sequences on the short arm of both chromosome 21 and other chromosomes. This new collection of probes homologous to chromosome 21 should facilitate molecular analyses of trisomy 21 by providing DNA probes for the linkage map of chromosome 21, for studies of nondisjunction, for chromosome walking in clinically relevant subregions of chromosome 21, and for the isolation of genes on chromosome 21 following the screening of cDNA libraries. |
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