Nociceptive reaction-related state of glial intermediate filaments in the brain of rats with hyperfunction of the thyroid gland: An ontogenetic aspect |
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Authors: | P A Nerush E M Demchenko |
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Institution: | (1) Dnepropetrovsk State Medical Academy, Ministry of Public Health of Ukraine, Ukraine |
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Abstract: | In experiments on Wistar rats of two age groups (5 weeks old and mature, 5 months old), we studied the content and polypeptide
composition of glial fibrillary acidic protein (GFAP) in the cerebral cortex, hippocampus, thalamus, and brainstem under conditions
of experimentally induced hyperfunction of the thyroid gland, as well as of combination of hyperthyroidism with the pain model
(consequences of laparotomy). The hyperthyroid state was created by administration of L-thyroxine (initial dose, 10 μg/day)
with food for 2 weeks; the dose was increased daily by 10 μg. At the end of experiment, we measured the level of thyroxine
in the blood serum using an immunoenzymatic technique. We found that in the hyperthyroid state 5-week-old rats showed a significant
increase in the content of filamentous GFAP fraction in the hippocampus, while when this influence was combined with the postoperation
pain syndrome, an increase was observed in the brainstem (by 18 to 27%). Results of immunoblotting demonstrated that degraded
polypeptides with a molecular mass of 47 to 45 kdalton were manifested under these conditions, which is indicative of intensification
of cytoskeletal rearrangement of astroglia. In mature rats under the same conditions, we observed a drop in the level of insoluble
polypeptides of intermediate filaments in astrocytes in the thalamus, hippocampus, and cerebral cortex.
Neirofiziologiya/Neurophysiology, Vol. 38, No. 4, pp. 280–286, July–August, 2006. |
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Keywords: | CNS hyperfunction of the thyroid gland emotional/painful state glial fibrillary acidic protein (GFAP) ontogenesis |
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