Improved lineshape and sensitivity in the HNCO-family of triple resonance experiments |
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Authors: | Daiwen Yang Lewis E. Kay |
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Affiliation: | (1) Protein Engineering Network of Centers of Excellence and the Departments of Medical and Molecular Genetics, Biochemistry and Chemistry, The University of Toronto, ON, Canada, M5S 1A8 |
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Abstract: | A simple scheme is presented for the suppression of dispersive contributions to cross peaks in HNCO-type spectra where the 15N chemical shift is recorded in a constant-time manner immediately prior to the transfer from 15 N to 1HN at the end of the sequence. These dispersive contributions arise when the delay for refocusing the 15N-13CO one-bond coupling is set to less than 0.5/1JN,CO and when 2 JHN,CO 0. Improvements in sensitivity in 1HN detected experiments recorded on 15 N,13C-labeled samples can be realized by application of 13CO/13 decoupling during acquisition. Sensitivity gains on the order of 15% and 5% have been obtained for an SH3 domain (62 residues) and maltose binding protein (370 residues), respectively. |
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Keywords: | absorptive lineshapes 13CO/13 IE2" > /content/T6356J073VQ11878/10858_2004_Article_234587_TeX2GIFIE2.gif" alt="
$${text{C}}^alpha$$
" align=" middle" border=" 0" > decoupling HNCO sensitivity enhancement |
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