Bioluminescent bioreporter integrated-circuit sensing of microbial volatile organic compounds |
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Authors: | S?Ripp K?A?Daumer T?McKnight L?H?Levine J?L?Garland M?L?Simpson Email author" target="_blank">G?S?SaylerEmail author |
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Institution: | (1) Center for Environmental Biotechnology, University of Tennessee, 676 Dabney Hall, Knoxville , TN 37996-1605, USA;(2) Dynamac Corporation, Kennedy Space Center, FL 32899, USA;(3) Molecular-Scale Engineering and Nanoscale Technologies Research Group, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA |
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Abstract: | A bioluminescent bioreporter for the detection of the microbial volatile organic compound p-cymene was constructed as a model sensor for the detection of metabolic by-products indicative of microbial growth. The bioreporter, designated Pseudomonas putida UT93, contains a Vibrio fischeri luxCDABE gene fused to a p-cymene/p-cumate-inducible promoter derived from the P. putida F1 cym operon. Exposure of strain UT93 to 0.02–850 ppm p-cymene produced self-generated bioluminescence in less than 1.5 h. Signals in response to specific volatile organic compounds (VOCs) such as m- and p-xylene and styrene, also occurred, but at two-fold lower bioluminescent levels. The bioreporter was interfaced with an integrated-circuit microluminometer to create a miniaturized hybrid sensor for remote monitoring of p-cymene signatures. This bioluminescent bioreporter integrated-circuit device was capable of detecting fungal presence within approximately 3.5 h of initial exposure to a culture of p-cymene-producing Penicillium roqueforti. |
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Keywords: | BBIC Bioluminescence Bioreporter lux MVOC p-cymene |
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