Isolation and identification of Sydowia polyspora and its pathogenicity on Pinus yunnanensis in Southwestern China |
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| Authors: | Yue Pan Hui Ye Jun Lu Peng Chen Xu‐Dong Zhou Min Qiao Ze‐Fen Yu |
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| Institution: | 1. Laboratory of Biological Invasion and Ecosecurity, Yunnan University, Kunming, Yunnan, China;2. Laboratory for Conservation and Utilization of Bio‐resources, Key Laboratory Resources of the Ministry of Education, Yunnan University, Kunming, Yunnan, China;3. Yunnan Academy of Forestry, Kunming, Yunnan, China;4. FuturaGene Biotechnology (Shanghai) Co., Ltd., Shanghai, China |
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| Abstract: | Sydowia polyspora occurs often as an epiphyte or endophyte of conifers and is widely distributed around the world. It is also reported as a fungus associated with bark beetles. In this study, based on ITS sequence data and morphological characteristics, 14 strains collected from the beetle bodies and galleries of Tomicus minor and Tomicus yunnanensis infesting Pinus yunnanensis in Southwestern China were identified as S. polyspora. To assess the virulence of S. polyspora to P. yunnanensis, pathogenicity tests were conducted by inoculating the fungus in the stems and on needles of P. yunnanensis. The results after 30 days showed that the lesion lengths in the phloem after inoculation with S. polyspora were significantly longer than for the control. In addition, the fungus caused partly distinct chlorisis or discolouration of needles 14 days after inoculation. This suggests that S. polyspora is able to colonize stem phloem and pine needles of P. yunnanensis and thus may cause secondary damages to this host after being spread with bark beetles. |
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| Keywords: | chlorotic and discoloured needles lesion phloem morphology phylogenesis |
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