| Affiliation: | (1) Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, 6205, Bangladesh;(2) Department of Bimolecular Engineering, Faculty of Bioscience and Biotechnology, T I T, Yokohama 226-8501, Japan |
| Abstract: | A mannose/glucose specific lectin have been isolated and purified from mulberry seeds by affinity chromatography on ConA Sepharose. The lectin is monomer in nature as judged by SDS-PAGE and its MW was estimated to be 22,000. The lectin is glycoprotein with neutral sugar content of 28.57%, and mannose and glucose were identified as carbohydrate. The lectin agglutinated rat red blood cells and in a hapten inhibition test, D-mannose and D-glucose was found to be inhibitor. The lectin also exhibited cytotoxic effect in brine shrimp lethality bioassay. The N-terminal sequences of the lectin upto 45-residues except the positions of 21, 39, 42 and 44 were identified. Sequence homology of the lectin is also discussed. |
