Single Step Purification,Characterization and N-Terminal Sequences of a Mannose Specific Lectin from Mulberry Seeds |
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Authors: | Email author" target="_blank">Nurul?AbsarEmail author T?Yeasmin M?Salim?Raza S?Kumar?Sarkar F?Arisaka |
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Institution: | (1) Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, 6205, Bangladesh;(2) Department of Bimolecular Engineering, Faculty of Bioscience and Biotechnology, T I T, Yokohama 226-8501, Japan |
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Abstract: | A mannose/glucose specific lectin have been isolated and purified from mulberry seeds by affinity chromatography on ConA Sepharose.
The lectin is monomer in nature as judged by SDS-PAGE and its MW was estimated to be 22,000. The lectin is glycoprotein with
neutral sugar content of 28.57%, and mannose and glucose were identified as carbohydrate. The lectin agglutinated rat red
blood cells and in a hapten inhibition test, D-mannose and D-glucose was found to be inhibitor. The lectin also exhibited cytotoxic effect in brine shrimp lethality bioassay. The N-terminal
sequences of the lectin upto 45-residues except the positions of 21, 39, 42 and 44 were identified. Sequence homology of the
lectin is also discussed. |
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Keywords: | Biological activity mulberry seed mannose specific lectin N-terminal sequences |
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