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Genetic diversity and genetic relationships in Hyacinthaceae in India using RAPD and SRAP markers
Authors:Tabassum Jehan  Amit Vashishtha  S R Yadav  Suman Lakhanpaul
Institution:1. Department of Botany, University of Delhi, Delhi, 110007, India
2. Department of Botany, Shivaji University, Kolhapur, Maharashtra, India
Abstract:Genetic diversity and relationship among three genera namely Drimia, Dipcadi and Ledebouria of Hyacinthaceae in India was studied using RAPD and SRAP markers. Twenty one RAPD primers and nine SRAP were used for analyzing 41 accessions. RAPD gave an average 12.6 markers per primer, while SRAP generated 10.1 markers per primer pair. The family emerged very diverged with high polymorphism. The study resolved the three genera into monophyletic groups corresponding to three subfamilies; Urginoideae, Hyacinthoideae and Ornithogaloideae. Drimia wightii emerged a very distinct species and species specific markers were obtained with both marker systems. AMOVA analysis also revealed the genera to be quite well diverged. The two markers showed high correlation (r?=?0.932) in Mantel matrix crresspondance test. The combined data also showed a very good correlation with the respective markers individually.
Keywords:Drimia  Dipcadi  Genetic diversity  SRAP  RAPD
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