Immortalized cell lines from murine embryos are characterized by progressive destabilization of their karyotype structure |
| |
Authors: | Grinchuk T M Pugovkina N A Tarunina M V Sorokina E A Ignatova T N |
| |
Affiliation: | Institute of Cytology RAS, St. Petersburg. gmdmk@mail.cytspb.rssi.ru |
| |
Abstract: | The karyotype structure was studied for three cell lines obtained from cells of transgenic murine embryos at early stages of their establishment. The first line was obtained from a transgenic embryonic explantant containing oncogen v-sis under promotor MMTV, two other lines originated from cells of transgenic embryos containing oncogen k51. The karyotypic analysis of G-banded metaphase chromosomes revealed deviations from the normal mouse karyotype as early as by the third passage of cultivation of independent embryonic cell lines that contained a foreign oncogene in their genome. The repeated analysis that involved 15-22 passages revealed similar abnormalities: variability and progression in chromosome number with the appearance of hyperpolyploid combinations, and a large number of rearranged chromosomes, both marker and unique ones. It is concluded that introduction of a foreign oncogene into murine cell genome leads to its enhanced and progressive non-specific destabilization. Oncogen v-sis produces a more valuable karyotype destabilization than oncogen k51. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|