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Cloning of a gene required for tryptophan biosynthesis from Leptospira biflexa serovar patoc into Escherichia coli |
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| Authors: | David B. Yelton Nyles W. Charon |
| Affiliation: | Department of Microbiology, West Virginia University Medical Center, Morgantown, WV 26506 U.S.A. Tel. (304) 293-2649 or 293-4170 |
| Abstract: | A clone bank, consisting of approx. 8100 colonies, has been created for the spirochete Leptospira biflexa serovar patoc in Escherichia coli using pBR322 as the vector. One of these clones contains the genetic information needed to complement a defect in the trpE gene of E. coli. The information resides on a 20.5-kb plasmid designated pYCl, which carries a 16-kb insert consisting of three HindIII fragments. It does not complement defects in other genes needed for the biosynthesis of tryptophan in E. coli. |
| Keywords: | Plasmid pBR322 recombinant DNA tryptophan biosynthesis Ap ampicillin EtBr ethidium bromide kb kilobase pairs restriction minus modification plus SDS sodium dodecyl sulfate SSC Tc tetracycline Trp tryptophan |
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