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Analysis of a 1963-bp polymorphic region flanking the human insulin gene |
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| Authors: | David Owerbach Lissi Aagaard |
| Affiliation: | 1. Department of Biochemistry, University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA 01605 U.S.A. Tel. (617) 856-5570;2. Hagedom Research Laboratory, Niels Steensensvej 6, DK2820, Gentofte Denmark Tel. (01) 68 08 60 |
| Abstract: | The nucleotide sequence of a long polymorphic region located 365 bp upstream from the human insulin gene is reported. The region is composed of 139 repeating sequences whose consensus structure is related to ACAGGGGTGTGGGG. Expansion in the number of repeating sequences appears to have taken place through duplication and triplication of 112–141-bp regions. However, ancestral polymorphic regions containing additions or deletions of 50 bp or more were not detected in two previous generations. |
| Keywords: | Recombinant DNA gene library nucleotide sequence phage β vectors genomic repeats restriction analysis bp base pairs Denhardt's solution 0.02% each bovine serum albumin, Ficoll 400, polyvinylpyrrolidone 360 kb 1000 bp SDS sodium dodecyl sulfate SSC To whom correspondence and reprint requests should be sent on request a detailed experimental evidence for conclusions reached in this short presentation will be supplied. |
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