Searle Research and Development, P. 0. Box 53, Lane End Road, High Wycombe, Buckinghamshire U.K.. Tel. (0494) 21124
Abstract:
A 43-bp DNA duplex coding for poly(arginine) poly(arg)] has been synthesised by modified phosphotriester procedures. It has been inserted into the BglII and BamHI restriction sites of a cloned synthetic β-urogastrone (Uro) gene, under the control of the trp promoter. Subsequent induction with 3β-indole acrylic acid produces β-Uro with a C-terminal poly(arg) fusion. The raised isoelectric point of this polypeptide fusion facilitates rapid purification by cation exchange chromatography. The C-terminal poly(arg) tail can be readily removed by treatment with carboxypeptidase B.