Novel biocatalytic process of N-substituted acrylamide synthesis |
| |
Authors: | K. V. Lavrov G. A. Larikova A. S. Yanenko |
| |
Affiliation: | 1. State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia
|
| |
Abstract: | The enzymatic synthesis of N-substituted acrylamides (N-isopropyl acrylamide and N, N-dimethylaminopropyl acrylamide) was demonstrated for the first time. The Rhodococcus erythropolis 37 strain, exhibiting acylamidase activity, was used as a source of enzyme, and water-dissolved acrylamide and isopropylamine/dimethylaminopropylamine served as substrates. The optimum conditions for the synthesis of acrylamide N-substitutes were determined using N-isopropyl acrylamide. The yield of the product was maximum at pH 9.5–10.5, substrate (acrylamide/isopropylamine) ratio within the range from 1.3: 1 to 2: 1, and absolute substrate concentrations of 8.0 (acrylamide) and 4.0% (isopropylamine). These conditions allowed for the synthesis of 22 g/L of N-isopropyl acrylamide. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|