Increased sister chromatid exchanges in human cell lines characterized by monosomy X or structural abnormalities of the X chromosome |
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Authors: | Mohammed A Iqbal Alice O Martin Joe Leigh Simpson |
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Institution: | (1) Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave., 10461 Bronx, NY, USA;(2) Section of Human Genetics, Department of Obstetrics and Gynecology, Northwestern University Medical School, 60611 Chicago, IL, USA |
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Abstract: | Summary In the present investigation we test the hypothesis that deficiencies in the X chromosome affect sister chromatid exchange (SCE) frequencies in human fibroblast cell lines. Our results show increased mean SCE frequencies in cell lines with abnormalities of the X chromosome: 45,X; 46,X,del(X) (q13), 46,X,del(X)(p11), and 46,X,i(Xq); control cell lines were 46,XX. In only one abnormal line 46,X,del(X)(p11)] was the increase not significant after correcting for multiple comparisons. If SCE formation is replication-dependent, the increased SCE frequencies might merely reflect the prolonged cell cycle we reported previously in cell lines with X chromosome abnormalities (Simpson and LeBeau 1981). Other explanations for differences between cell lines are possible, e.g., that deleted loci on the X chromosome affect cellular uptake of bromodeoxyuridine (BrDU). However, specific mechanisms were not explored directly. |
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