High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans |
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| Authors: | Lee Siu F White Vanessa L Weeks Andrew R Hoffmann Ary A Endersby Nancy M |
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| Institution: | Department of Genetics, Bio21 Institute, The University of Melbourne, Victoria, Australia. ronaldl@unimelb.edu.au |
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| Abstract: | We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening. |
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