Determination of free and conjugated bile acids as ultraviolet-absorbing ion pairs by reverse-phase high-performance liquid chromatography. |
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| Authors: | N Parris |
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| Institution: | Eastern Regional Research Center, Agriculture Research, Science and Education Administration, U. S. Department of Agriculture, Philadelphia, Pennsylvania 19118, USA |
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| Abstract: | A method for the determination of free and conjugated bile acids as uv-absorbing ion pairs was developed. Ultraviolet photometric detection was more sensitive than differential refractometer detection. Improved resolution of positional isomers was also achieved. Distinctions were made between free and conjugated bile acids and between tauro- and glyco-conjugated bile acids. This was accomplished by adjusting the pH of the mobile phase to selectively form ion pairs. |
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