| 人血清中膦酸单酯酶性质的研究 |
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| 引用本文： | 韩刚毅,范西红,靳仙宝,唐咏,王东沛,单礼成.人血清中膦酸单酯酶性质的研究[J].中国生物化学与分子生物学报,1991,7(1):83-88. |
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| 作者姓名： | 韩刚毅 范西红 靳仙宝 唐咏 王东沛 单礼成 |
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| 作者单位： | 济南军区军事医学研究所,济南军区总医院,山东省千佛山医院,济南军区军事医学研究所,济南军区军事医学研究所,济南军区总医院 济南 250014,济南,济南,济南 250014,济南 250014,济南 |
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| 摘 要： | 本文采用4-甲基伞形酮苯基磷酸酯为底物检测人血清中酶活性情况,发现在人血清中存在一种能够选择性水解苯基膦酸单酯键的酶活性成份。该酶具有最适pH8.8—9.1,在60℃(反应30分钟)条件下具有最大活性。Km=1.72×10~(-4)mol/L,Na_3PO_4、EDTA和半胱氨酸可抑制其活性,而CuSO_4、腺苷、胸苷、NaN_3、E600、PCMB、DFP和毒扁豆碱等对其活性没有影响。Mg~(++)可激活酶活性,并能解除EDTA的抑制作用。 此酶不能水解5′-NPDase和APase的底物,有关性质也与5′-NPDase和APasc有区别。本文将此酶暂定名为“膦酸单酯酶”。
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| 关 键 词： | 磷酸二酯酶Ⅰ 磷酸单酯酶 5′—核苷酸磷酸二酯酶 碱性磷酸酶 人血清 |
| 收稿时间： | 1991-02-20 |
Detection and Property of A Specific Phosphonate Monoesterase in Human Serum |
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| Han,Gang-yi Fan,Xi-hong Jin,Xian-bao Tang,YiungWang,Dong-pei Shan,Li-cheng.Detection and Property of A Specific Phosphonate Monoesterase in Human Serum[J].Chinese Journal of Biochemistry and Molecular Biology,1991,7(1):83-88. |
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| Authors: | Han Gang-yi Fan Xi-hong Jin Xian-bao Tang YiungWang Dong-pei Shan Li-cheng |
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| Institution: | (Jinan Instiute of Military Medicine Jinan, Shandong 250014 |
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| Abstract: | Enzyme capable of hydrolyzing the 4-methylumbelliferyl phenylphos-phonate to 4-methylumbellif erone and phenylphosphonic acid has been detected in human serum. It has Km at 1.72 × 10-4mol/L., with optimum pH of 8.8-9.1 and maxium activity at 60℃(30 min). The enzymic activity can be inhibited by Na3PO4 EDTA and Cysteine but activited by Mg2+. No effect of CuSO4、 NaN3、E600、PCMB、DFP and Eserine on the enzymic activity was found. It can not hydrolyze substrates for 5'-nucleotide phosphodiesterase and alkaline phosphatase. The enzyme was considered as "Phosphonate monoesterase" |
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| Keywords: | Phosphonate monoesterase Phosphodiesterase I 5' -Nucleotide phos-phodiesterase Alkaline phosphatase |
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