A novel nuclear factor, SREB, binds to a cis-acting element, SRE, required for inducible expression of the Aspergillus oryzae Taka-amylase A gene in A. nidulans |
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Authors: | Tani S Kawaguchi T Kato M Kobayashi T Tsukagoshi N |
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Institution: | (1) Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya-shi, Aichi 464-8601, Japan E-mail: koba@agr.nagoya-u.ac.jp Tel.: +81-52-7894086; Fax: +81-52-7894087, JP |
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Abstract: | The Taka-amylase A gene (taaG2) of Aspergillus oryzae is inducibly expressed in A. nidulans upon exposure to inducing carbon sources, such as starch and maltose. In order to identify nuclear factor(s) possibly involved
in the induction of the taaG2 gene, gel mobility shift assays and DNase I footprinting analyses were carried out, and revealed a novel nuclear factor in
A. nidulans extracts, which specifically bound to two sites in the taaG2 promoter region, −204 to −189 and −182 to −168, which share the common sequence GGAAATT. The nuclear factor was detected
in nuclei from both induced and uninduced mycelia. Mutational analysis within and around the binding sequences demonstrated
that only the upstream binding sequence, designated SRE (starch responsive element), was required for the inducible expression
of the taaG2 gene, and thus we designated the nuclear factor SREB (SRE binding factor). The downstream binding site contained an inverted
SRE (ISRE) and played no role in the induction of taaG2 expression. SREB was shown by gel retardation assays to have higher affinity for SRE than for ISRE.
Received: 26 January 1999 / Accepted: 10 November 1999 |
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Keywords: | Taka-amylase A gene Aspergillus nidulans Gene regulation Nuclear factor Cis-acting element |
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