Removal of phenolics from plant extracts by grinding with anion exchange resin |
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| Authors: | T H Lam M Shaw |
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| Affiliation: | 1. Department of Chemistry, Faculty of Science, Hail University, Hail, Saudi Arabia;2. Department of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia;3. Department of Chemistry, Faculty of Science, Taibah University, Al-Madinah Al-Minawara, Saudi Arabia;1. Dr. Ira Nimdeokar Postgraduate and Research Centre for Chemistry, Hislop College, Nagpur, Maharashtra 440002, India;2. Department of Chemistry, Government V.Y.T. Postgraduate Autonomous College, Durg, Chhattisgarh 491001, India;3. School of Biological and Chemical Sciences, Faculty of Science, Technology and Environment, The University of the South Pacific, Private Mail Bag, Suva, Fiji;4. Department of Natural Sciences, Faculty of Knowledge Engineering, Tokyo City University, Tokyo 158-8557, Japan |
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| Abstract: | A new method is described for removing phenolics during the preparation of crude plant extracts. Tissue was ground in Tris-glycine buffer with insoluble polyvinyl-pyrolidone (PVP) or Dowex 1-X8 anion exchange resin. Assays for peroxidase, o-diphenol oxidase, total protein and total phenolics showed that the resin removed interfering phenolics far more effectively than did PVP. |
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