热应激对小鼠器官指数、小肠损伤及胃HSP70 mRNA表达的影响

目的探讨热应激对小鼠器官指数、小肠形态、胃黏膜HSP70 mRNA表达量及糖代谢相关激素的影响。方法采用单因子实验设计,将年龄和体重相近的18只KM小鼠随机分为对照组和热应激组,分别测定心、肝、脾、肺、肾重量,小鼠胃黏膜HSP70 mRNA表达量、血浆中胰岛素和胰高血糖素浓度以及十二指肠和空肠的绒毛高度、隐窝深度,并对肝脏、十二指肠和空肠进行病理组织学检查。结果与结论热应激对小鼠器官指数无影响,可显著提高小鼠胃黏膜HSP70 mRNA表达量,降低血浆中胰岛素的含量,并造成小鼠肝脏、十二指肠和空肠严重损伤。

Effect of heat stress on the organ indices,small intestine damages and expression of HSP70 mRNA in the gastric mucosa of mice

Objective To investigate the effect of heat stress on organ indices, intestinal morphology, gastric mu-cosal HSP70 mRNA expression and glucose metabolic hormones in mice.Methods A single-factor experiment was de-signed for the present research.Eighteen KM mice of the similar age and weight were randomly divided into control group and heat stress group.The weight of the heart, liver, spleen, lung and kidney, as well as the expression of HSP70 mRNA in the mouse gastric mucosa were measured.The plasma concentration of insulin and glucagon, the villus height and crypt depth of the duodenum and jejunum were detected.The histological changes of the liver, duodenum and jejunum were also examined.Results There was no effect of heat stress on the organ indices.It significantly increased the expression of HSP70 mRNA in the gastric mucosa, reduced the plasma insulin level and caused serious injury to the liver, duodenum and jejunum in the mice.Conclusions Heat stress does not significantly affect the organ indices in mice, but can significantly increase the expression of HSP70 mRNA in the gastric mucosa, cause apparent damages in the liver, duodenum and jeju-num, reduce the villus height, crypt depth and villus height/crypt depth ratio of the duodenum and jejunum, and also de-crease the blood insulin concentration in the mice.