In vitro interactions between Neoparamoeba spp. and salmonid leucocytes; the effect of parasite sonicate on anterior kidney leucocyte function |
| |
| Authors: | K Gross † S Alcorn ‡ A Murray § R Morrison B Nowak |
| |
| Institution: | School of Aquaculture, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania and Aquafin CRC, Locked Bag 1370, Launceston, Tasmania 7250, Australia; School of Aquatic and Fishery Sciences, University of Washington, Box 355020, Seattle, WA 98195, U.S.A.; and Western Fisheries Research Centre, Biological Resources Division, United States Geological Survey, 6505 N.E. 65th Street, Seattle, WA 98115, U.S.A. |
| |
| Abstract: | Sonicated Neoparamoeba spp. (Nspp) did not affect the in vitro respiratory burst response of leucocytes isolated from Atlantic salmon Salmo salar , rainbow trout Oncorhynchus mykiss and chinook salmon Oncorhynchus tshawytscha anterior kidneys ( P > 0·05). Atlantic salmon and chinook salmon leucocytes pre-incubated with the parasites, however, responded to phorbol myristate acetate (PMA) stimulation with a greater response compared to cells incubated with PMA on its own ( P < 0·05). Sonicated Nspp was not chemo-attractive for anterior kidney leucocytes isolated from all three fish species. |
| |
| Keywords: | amoebic gill disease chemotaxis leucocyte Neoparamoeba spp phagocyte function respiratory burst |
|
|
