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Gluconobacter in biosensors: applications of whole cells and enzymes isolated from gluconobacter and acetobacter to biosensor construction
Authors:Juraj Švitel  Jan Tkáč  Igor Voštiar  Marian Navrátil  Vladimír Štefuca  Marek Bučko  Peter Gemeiner
Institution:(1) Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK 812 37 Bratislava, Slovakia;(2) Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK 845 38 Bratislava, Slovakia;(3) Cell Biology and Biochemistry, Pacific Northwest National Laboratories, 902 Battelle Blvd., Richland, WA 99354, USA;(4) Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK 812 37 Bratislava, Slovakia;(5) Present address: Department of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA
Abstract:Bacteria belonging to the genus Acetobacter and Gluconobacter, and enzymes isolated from them, have been extensively used for biosensor construction in the last decade. Bacteria used as a biocatalyst are easy to prepare and use in amperometric biosensors. They contain multiple enzyme activities otherwise not available commercially. The range of compounds analyzable by Gluconobacter biosensors includes: mono- and poly-alcohols, multiple aldoses and ketoses, several disaccharides, triacylglycerols, and complex parameters like utilizable saccharides or biological O2 demand. Here, the recent trends in Gluconobacter biosensors and current practical applications are summarized. An erratum to this article can be found at
Keywords:Acetobacter            Biosensor  Dehydrogenase  Enzymes            Gluconobacter            Whole cells
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