Restoration of myo-inositol uptake by eicosapentaenoic acid in human skin fibroblasts cultured in high-glucose medium |
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| Institution: | 1. Department of Dermatology, Saint-Luc University Hospital, Université catholique de Louvain, Brussels, Belgium;2. Institute of Experimental and Clinical Research, Pole of Pneumology, ENT and Dermatology, Université catholique de Louvain, Brussels, Belgium;3. Department of Allergy and Clinical Immunology, Hospital Center Lyon Sud, Lyon, France;4. CIRI- INSERM U1111 - CNRS UMR5308, Université Lyon 1, Université de Lyon, Lyon, France;1. Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China;2. Beijing Key Laboratory of Clinic and Basic Research with Traditional Chinese Medicine on Psoriasis, Beijing 100010, China;3. Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China;4. Beijing University of Chinese Medicine, Beijing 100029, China;1. The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Brisbane, Queensland, Australia;2. Department of Otolaryngology–Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China |
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| Abstract: | The effects of elevated glucose and eicosapentaenoic acid (EPA, C20:5 ω3) on myo-inositol uptake in human skin fibroblasts (HSF) were evaluated. Myo-inositol incorporation into HSF was dependent on an active transport system via Na+-K+ ATPase activity based on the results with Na+ deprivation and ouabain (5 mM). Although glucose (27.5, 55 mM) inhibited 2-3H] myo-inositol uptake, the addition of EPA (3×10?4M) prevented glucose-mediated inhibition. Since EPA decreased glucose-mediated inhibition of myo-inositol uptake, this agent might ameliorate some of the devastating functions associated with diabetes. |
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