Inhaled bordetella pertussis vaccine decreases airway responsiveness in guinea pigs |
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| Institution: | 1. Medical Laboratory, Mianyang 404 Hospital, Mianyang 621000, Sichuan, China;;2. School of Environmental Resource and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China;1. Institute of Immunology, Hannover Medical School, Germany;2. Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Germany;3. Department of Nephrology and Hypertension, Hannover Medical School, Germany;4. Department of Pneumology and German Centre of Lung Research (DZL), Hannover Medical School, Germany;5. Centre for Individualised Infection Medicine (CiiM), Hannover, Germany;6. Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany;7. Department of Rheumatology and Immunology, Hannover Medical School, Germany;8. Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Germany;9. Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, Germany |
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| Abstract: | Bordetella pertussis (BP) has been used as adjuvant for experimental animal immunization, but its effects on airway responsiveness are uncertain. Three groups of guinea pigs were used: animals with a single exposure to inhaled BP vaccine (strain 134, total dose 1.24 × 1012germs), animals submitted to a sensitization procedure through inhalation of ovalbumin plus BP and healthy control animals. Four weeks after inhalation of BP or after the beginning of sensitization, dose- or concentration-response curves to histamine were constructed in vivo and in vitro (tracheal and parenchymal preparations). We found that BP alone produced lower responses to histamine than control guinea pigs in vivo (insufflation pressure, p = 0.0003) and in tracheal tissues (p = 0.04), but not in parenchymal preparations. Sensitization did not modify the responsiveness compared with their respective controls. These results suggest that some BP component(s), probably pertussis toxin, causes a long lasting airway hyporesponsiveness in guinea pigs. |
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