| N-乙酰氨基葡萄糖苷内切酶的功能与机制 |
| |
| 引用本文： | 芦鑫荣,童永亮,孔维溧,邹琳,沈旦枫,吕绍衔,刘瑞杰,张绍兴,张瑜芯,侯琳琳,孙桂芹,陈力.N-乙酰氨基葡萄糖苷内切酶的功能与机制[J].生物化学与生物物理进展,2024,51(5):985-999. |
| |
| 作者姓名： | 芦鑫荣 童永亮 孔维溧 邹琳 沈旦枫 吕绍衔 刘瑞杰 张绍兴 张瑜芯 侯琳琳 孙桂芹 陈力 |
| |
| 作者单位： | 1)复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生健康委员会重点实验室,上海 200032,1)复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生健康委员会重点实验室,上海 200032,1)复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生健康委员会重点实验室,上海 200032,1)复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生健康委员会重点实验室,上海 200032,1)复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生健康委员会重点实验室,上海 200032,1)复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生健康委员会重点实验室,上海 200032,2)浙江中医药大学医学技术与信息工程学院,杭州 310053,2)浙江中医药大学医学技术与信息工程学院,杭州 310053,2)浙江中医药大学医学技术与信息工程学院,杭州 310053,3)青岛农业大学动物医学院,青岛 266106,2)浙江中医药大学医学技术与信息工程学院,杭州 310053,1)复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生健康委员会重点实验室,上海 200032 |
| |
| 基金项目： | 国家重点研发计划（2021YFC2700801） 资助项目。 |
| |
| 摘 要： | N-乙酰氨基葡萄糖苷内切酶（endo-beta-N-acetylglucosaminidase,ENGase）广泛分布于各种生物中，主要通过降解错误折叠的糖蛋白，参与细胞和生命的调控。ENGase也是糖链编辑的有效工具酶，可专一性水解游离寡糖链及糖肽或糖蛋白上核心五糖的N-乙酰氨基葡萄糖（GlcNAc）之间的β-1,4糖苷键。其水解产物是寡糖链和一个GlcNAc，或带有一个GlcNAc的糖肽或糖蛋白。本文对ENGase的发现、分布、蛋白质结构、酶学反应及生物学功能进行阐述，为ENGase的生物学研究提供思路，为糖生物学与糖组学的应用研究奠定基础。
|
| 关 键 词： | N-乙酰氨基葡萄糖苷内切酶 N-糖基化 游离寡糖 糖链编辑 糖生物学 |
| 收稿时间： | 2023/7/9 0:00:00 |
| 修稿时间： | 2024/4/8 0:00:00 |
Endo-beta-N-acetylglucosaminidase: Possible Functions and Mechanisms |
| |
| Institution: | 1)Key Laboratory of Medical Molecular Virology of Ministries of Education and National Health Committee, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China,1)Key Laboratory of Medical Molecular Virology of Ministries of Education and National Health Committee, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China,1)Key Laboratory of Medical Molecular Virology of Ministries of Education and National Health Committee, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China,1)Key Laboratory of Medical Molecular Virology of Ministries of Education and National Health Committee, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China,1)Key Laboratory of Medical Molecular Virology of Ministries of Education and National Health Committee, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China,1)Key Laboratory of Medical Molecular Virology of Ministries of Education and National Health Committee, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China,2)School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China,2)School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China,2)School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China,3)College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266106, China,2)School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China,1)Key Laboratory of Medical Molecular Virology of Ministries of Education and National Health Committee, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China |
| |
| Abstract: | |
| |
| Keywords: | ENGase N-glycosylation free oligosaccharides (fOSs) glycan editing glycobiology |
|
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
