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Utilization of molasses for the production of fat by an oleaginous yeast,Rhodotorula glutinis IIP-30
Authors:Von W. Johnson  Manjit Singh  Virender S. Saini  Dilip K. Adhikari  Venkatrao Sista  Natwarsinh K. Yadav
Affiliation:(1) Microbiology Dept, School of Sciences, Gujarat University, 380 009 Ahmedabad, India;(2) Research Centre, Indian Petrochemicals Corporation Ltd, 391 345 Baroda, India;(3) Indian Institute of Petroleum, 248 005 Dehradun, India;(4) Present address: Biotechnology Laboratory, Research Centre, Gujarat State Fertilizers Company Ltd, 391 750 Baroda, India;(5) Present address: Pfizer Ltd, 178 Industrial Area, 160 002 Chandigarh, India
Abstract:Summary Rhodotorula glutinis is known to produce fat when cultivated under nitrogen-limiting conditions. Economically, molasses is an ideal substrate, however, due to the presence of nitrogen in molasses, the lipid yield obtained is much lower than that obtained from glucose or sucrose. Higher yields were obtained using molasses in a fed batch fermentation supplemented with glucose or sucrose during the lipid accumulation phase. The fatty acids profile of the lipids thus produced, using a very simple and economical medium, was similar to that obtained from glucose and sucrose.
Keywords:Yeast  Oleaginous  Rhodotorula glutinis  Lipid  Fat  Molasses  Fed batch
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