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GalNAc pretreatment inhibits trapping of Bacillus thuringiensis Cry1Ac on the peritrophic membrane of Bombyx mori |
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| Authors: | Hayakawa Tohru Shitomi Yasuyuki Miyamoto Kazuhisa Hori Hidetaka |
| Institution: | Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan. |
| Abstract: | Bombyx mori (ShunreixShogetsu) is sensitive to Cry1Aa and resistant to Cry1Ac, both insecticidal proteins of Bacillus thuringiensis. Cry1Aa passed through the peritrophic membrane (PM) much faster (0.37 microg/mm2 PM/h) than Cry1Ac (0.05 microg/mm2 PM/h) during the initial observation period. Both Cry1Aa and Cry1Ac bound to the PM but only the binding of Cry1Ac was specifically inhibited by N-acetylgalactosamine (GalNAc). When Cry1Ac was pretreated with GalNAc, Cry1Ac permeated the PM much faster. These results suggested that Cry1Ac bound a PM protein via GalNAc on a sugar side chain. The role of the PM on Cry1Ac resistance of B. mori was briefly discussed. |
| Keywords: | PM peritrophic membrane GalNAc N-acetylgalactosamine BBM(V) brush border membrane (vesicle) of the larval midgut Cry insecticidal toxin of Bacillus thuringiensis APN aminopeptidase N CA carbonic anhydrase |
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