水貂自咬症个体分子鉴定引物的开发与应用

<正>自咬症是危害笼养水貂(Mustla vison)的一种慢性疾病,其特征是阵发性神经高度兴奋、反复自咬身体后躯和尾部、对外界刺激敏感。常因外界刺激引起高度兴奋发作,干扰兽群繁殖。若咬伤部

Development and application of primers for molecular identification of self-biting individuals among minks

DNA samples obtained from healthy and self-biting Jinzhou Black minks were amplified by PCR with 100 random primers. A DNA fragment corresponding to self-biting cases was identified and marked as S356. The DNA fragment was purified and cloned into PMD18-T vector, subsequently, transformed into E. Coli JM109 and sequenced. We applied the SCAR label technology to detect the molecular marker S356 among the healthy and self-biting minks. Based on the sequence of S356, two pairs of SCAR primers,S356 - 8001 and S356 - 8002,were designed and used in PCR detection. The result showed that the target DNA fragment was amplified from 29 of 30 self-biting minks (96.67% positive correspondence rate) with the SCAR labeling primers S356 - 8001 and S356 -8002. However, there was no target fragment detected from healthy minks. The DNA fragment was denominated as DS356800, which could be used as a genetic marker to differentiate potential self-biting individuals from healthy minks, and could form a guideline for future anti-disease genetic breeding to eliminate the self-biting disease from mink populations.