| DNA分子量标准制备技术：方法与进展 |
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| 引用本文： | 张颖,刘发珍,李强,叶春江.DNA分子量标准制备技术：方法与进展[J].中国生物工程杂志,2009,29(8):119-123. |
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| 作者姓名： | 张颖 刘发珍 李强 叶春江 |
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| 作者单位： | 1.济南大学 医学与生命科学学院 生物科学与技术系 济南 2500222
2.山东大学 微生物技术国家重点实验室 济南 250100 |
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| 摘 要： | DNA分子量标准是一组分子量大小已知的DNA片段混合物，用于指示核酸电泳中未知样品的分子量大小，从而帮助实验人员判断DNA样品的性质。因而DNA分子量标准成为目前分子生物学和基因工程领域不可或缺的一种电泳耗材。综述了目前各种DNA分子量标准产品的制备方法和技术原理及近年来该领域的一些技术进展情况。
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| 关 键 词： | DNA分子量标准 载体构建 PCR扩增 限制性内切酶酶切 |
| 收稿时间： | 2009-04-03 |
| 修稿时间： | 2009-04-27 |
Preparation of DNA Molecular Weight Standards: Methods and Advances |
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| ZHANG Ying,LIU Fa-zhen,LI Qiang,YE Chun-jiang.Preparation of DNA Molecular Weight Standards: Methods and Advances[J].China Biotechnology,2009,29(8):119-123. |
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| Authors: | ZHANG Ying LIU Fa-zhen LI Qiang YE Chun-jiang |
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| Institution: | 1.Department of Bioscience and Biotechnology, College of Medicine and Life Sciences, University of Jinan, Jinan250022, China
2.State Key Laboratory of Microbial Technology, College of Life Science, Shandong University, Jinan250100, China |
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| Abstract: | DNA molecular weight standards are a mixture of a grou Pof DNA molecules with known sizes to reference the molecular weight (mass) of unknown DNA samples in nucleic acid electrophoresis and hel Pthe experimentalists to judge the property of the DNA samples. At the present time the DNA molecular weight standards are essential for nucleic acid electrophoresis analysis in the molecular biology and genetic engineering fields. The methods and principles for the production of DNA molecular weight standards with the recent advances in this field were summarized. |
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| Keywords: | DNA molecular weight standards Vector construction PCR technology Restrictive digestion |
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