Comparative study of appearance of water-soluble antigens and brain esterase fractions in ontogenesis of two strains of rats

By means of immunoelectrophoresis of rat brain, 12 water-soluble antigens were detected, five of which were found to be specific to the brain. Histochemical reactions have identified two antigens that are not specific to the brain, lactate dehydrogenase and esterase. By means of enzymoelectrophoresis, 14 esterase fractions were determined. An immunoautoradiographic study of the synthesis of some antigens specific to the brain was carried out. It was found that rats responding to sound by epileptic seizures develop more slowly than normal rats. In particular, in rats resistant to auditory stimulus the antigenic spectrum typical of the brain of the adult rat (12 antigens) is formed by day 14 of postnatal life, while in rats of the sensitive strain this pattern takes 17 days to completely form. The last brain-specific antigen for rats susceptible to audiogenic epilepsy appears on day 17, while this antigen is present in normal rats on day 14. Fast-moving esterase fractions are detected earlier in ontogenesis in rats resistant to sound than in sensitive rats.