Cell volume changes during rapid temperature shifts |
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Authors: | Gervais Patrick Martínez de Marañon Iñigo Evrard Christine Ferret Eric Moundanga Sylvie |
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Institution: | Laboratoire de Génie des Procédés Alimentaires et Biotechnologiques, ENSBANA, 21000 Dijon, France. gervais@u-bourgogne.fr |
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Abstract: | The effect of a rapid temperature increase on the volume of different types of cells was investigated. Experiments were carried out using continuous microscopic image analysis. Volume variation of yeast cells, yeast spheroplasts and human leukaemia cells was measured during the transient phase after a thermal shift. The thermal shift was found to induce rapid increase in cell volume for cells lacking a cell wall (yeast spheroplasts and human leukaemia cells). This increase in cell volume is assumed to be a main cause of the heat shock-induced cell death. A theoretical mechanistic model that explains the behaviour of these cells is finally proposed. |
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